Israeli billionaire Teddy Sagi previously sold a stake in Playtech.
Mr Sagi will sell 13m shares – representing 4.1 per cent of Playtech – at 872.5 pence to an undisclosed institutional investor for a sum in excess of £133m, a statement on behalf of the entrepreneur said.
The Playtech founder made the sale as part of his plans to further diversify into London property, co-working and e-commerce, he said in the statement.
"Playtech is a company which I believe will continue to achieve significant growth over the coming years," Mr Sagi commented.
Mr Sagi previously sold a stake in Playtech, which is one of the world’s largest supplier of software and services to the online gaming industry, in November. The current sale will leave the billionaire with a 17.8 per cent stake in the company.
Playtech said last month that it planned to continue its acquisition spree this year on the back of continued strong revenue growth.
The company, which is sitting on a cash pile of €545m, bought Australian bingo software group Eyecon for £50m in February and snapped up rival operator Best Gaming Technology for €138m last year.